T7 RNA Polymerase antibody

Catalog No. ABIN7540668

Supplier Product No.: 200-401-mx5

The Rabbit Polyclonal anti-T7 RNA Polymerase antibody is suitable to detect T7 RNA Polymerase in samples from Bacteriophage T7. It has been validated for ELISA and WB.

Quick Overview for T7 RNA Polymerase antibody (ABIN7540668)

Target: T7 RNA Polymerase

Reactivity: Bacteriophage T7

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Supplier Product No.: 200-401-mx5

Supplier: Rockland

Purpose: T7 RNA Polymerase Antibody

Cross-Reactivity (Details): Protein A purified T7 RNA Polymerase antibody is directed against T7 Polymerase (E.coli)

Sterility: Sterile filtered

Immunogen: Anti-T7 Polymerase antibody was produced from whole rabbit serum produced by repeated immunizations with T7 Polymerase isolated from E. coli.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:11,000-1:13,000

Western_Blot_Dilution: 1.0 μg/mL

Comment:

This protein A purified Anti-T7 RNA Polymerase antibody has been tested for use in ELISA and Western Blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 98.9 kDa in size corresponding to T7 Polymerase by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: T7 RNA Polymerase

Background: Escherichia phage T7 (Bacteriophage T7), DNA-directed RNA polymerase, DNA-dependent RNA polymerase, RNA nucleotidyltransferase (DNA-directed),T7 RNA Polymerase is a highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. It recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. T7 RNA Polymerase unwinds the double-stranded DNA to expose the coding strand for templating. It participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Also, T7 RNA Polymerase plays a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process. In eukaryotes three forms of this enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized.

Gene ID: 1261050

NCBI Accession: NP_041960

UniProt: P00573