Recombinant SIRPA antibody

Catalog No. ABIN7542990

The Mouse Monoclonal anti-SIRPA antibody has been validated for WB, ELISA, IHC, IP, IF and Func. It is suitable to detect SIRPA in samples from Rat.

Quick Overview for Recombinant SIRPA antibody (ABIN7542990)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (Func)

Clone: OX-41

Product Details anti-SIRPA Antibody

Purpose: Anti-CD172A/SIRPA [OX-41], Mouse IgG2a, kappa

Specificity: This antibody binds to rat SIRP alpha, which is selectively expressed by myeloid and neuronal cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: Rat resident peritoneal cells injected into BALB/c mice.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody binds to SIRP alpha, and can consequently be used to label neuronal cells and myeloid cells, namely macrophages, monocytes, granulocutes and dendritic cells, in immunofluorescence and immunohistochemical studies (Robinson, 1986). Anti-SIRP ligation induces nitric oxide synthesis and oxidative burst in macrophages (Adams, 1998).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172A/SIRPA

Background: SIRPA, BIT, MFR, MYD-1, MYD1, P84, PTPNS1, SHPS1, SIRP, Signal-regulatory protein alpha, signal regulatory protein alpha, OX41, MRC OX-41, MRC OX 41

UniProt: P97710