Recombinant CD45RC antibody (Extracellular Domain)

Catalog No. ABIN7543009

The Rabbit Chimeric anti-CD45RC antibody (Clone OX-22) (ABIN7543009) specifically detects CD45RC in FACS, IP and IHC. The antibody is reactive with Rat samples.

Quick Overview for Recombinant CD45RC antibody (Extracellular Domain) (ABIN7543009)

Target: CD45RC

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD45RC antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: OX-22

Product Details anti-CD45RC Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-CD45RC [OX-22], Rabbit IgG, kappa

Specificity:

OX-22 binds specifically to the high molecular weight form of the leucocyte common antigen, CD45RC. OX-22 and OX-31 compete for binding but do not compete with OX-32. The antigen is expresssed on the surface of B cells, around half of bone marrow cells, all CD8+ve T cells, and is able to differentiate between CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like) cell types. CD45 extracellular domain is able to interact with a number of different proteins including CD4, TCR, CD2, Thy-1 and CD26. CD45 is important for signalling throguh the T cell receptor and in the selection of T and B lymphocytes.

Application Notes original Clone: OX-20 can be used for both FC and IHC (paraffin/frozen) studies. It can also be used to purify proteins by affinty-purification (Woolett et al. Eur J Immunol. 1985 Feb,15(2):168-73).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: OX-22 was prepared by immunizing BALB/c mice with phytohemagglutinin-activated rat lymph node cells.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD45RC

Target: CD45RC

Alternative Name: CD45RC

Background: L-CA, Receptor-type tyrosine-protein phosphatase C, leukocyte common antigen, T200, CD45, OX22, MRC OX22, MRC OX-22, CD45 R

UniProt: P04157