Recombinant CD20 antibody

Catalog No. ABIN7543095

The Rabbit Chimeric anti-CD20 antibody has been validated for Dep and St. It is suitable to detect CD20 in samples from Human.

Quick Overview for Recombinant CD20 antibody (ABIN7543095)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD20 antibody is un-conjugated

Application: Depletion (Dep), Stimulation (St)

Clone: 1F5

Product Details anti-CD20 Antibody

Purpose: Anti-CD20 [1F5], Rabbit IgG, kappa

Specificity: This antibody is specific for CD20, which is expressed by normal and malignant B cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by hyperimmunising BALB/c mice with the Raji Burkett's lymphoma line and the NALM-6 pre B leukaemia line.

Isotype: IgG kappa

Application Details

Application Notes: This antibody, either alone or in combination with T cell derived factors, induces B cell proliferation via CD20 cross-linking (Clark et al, 1985).When administered to patients with refractory malignant B cell lymphomas, this antibody depletes circulating and lymph node tumour cells, with minimal toxicity (Press et al, 1987). A murine/human chimeric IgG1 construct of this antibody has been shown to lyse Burkitt's lymphoma (5RAJI) cells (Stockmeyer et al, 2000), while IgG1 and IgA2 isotypes of this antibody have been shown to kill B cells in vitro and in deplete B cells in vivo (Lohse et al, 2018). The humanized version of this antibody can be provided upon request.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Background: MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, E9PPL6, P19437

UniProt: P11836