Recombinant CD20 antibody

Catalog No. ABIN7543096

This anti-CD20 antibody is a Rabbit Chimeric antibody detecting CD20 in FACS, IF and IP. Suitable for Human.

Quick Overview for Recombinant CD20 antibody (ABIN7543096)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD20 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 2H7

Product Details anti-CD20 Antibody

Purpose: Anti-CD20 [2H7], Rabbit IgG, kappa

Specificity: This antibody is specific for CD20, which is expressed by normal and malignant B cells.

Cross-Reactivity: Baboon, Chimpanzee, Gibbon, Marmoset, Owl Monkey, Primate (Semnopithecus)

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was raised by hyperimmunising a C57BL/6 mouse with the Ia- B cell line 6.16 cl. 3.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in flow cytometry and quantitative immunofluorescence analysis of human peripheral blood mononuclear leukocytes (Yanase et al, 1986).It has also been used to precipitate CD20 from lysates of the Raji human B cell line (Deans et al, 1995). This antibody has been exploited as part of a an avidin-biotin immunoadsorption system to eliminate Daudi lymphoblasts from human bone marrow (Berenson et al, 1986). A mouse-human chimeric version of this antibody effectively mediates antibody-dependent cellular cytotoxicity with human effector cells and complement-dependent cytotoxicity with human complement (Liu et al, 1987).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Background: MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, E9PPL6, P19437

UniProt: P11836