Recombinant CD20 antibody

Catalog No. ABIN7543098

The Human Chimeric anti-CD20 antibody has been validated for WB. It is suitable to detect CD20 in samples from Human.

Quick Overview for Recombinant CD20 antibody (ABIN7543098)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Human

Clonality: Chimeric

Conjugate: This CD20 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: B9E9

Product Details anti-CD20 Antibody

Purpose: Anti-CD20 [B9E9], Human IgG1, kappa

Specificity: This antibody is specific for CD20, which is expressed by normal and malignant B cells. It does not cross-react with murine CD20.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised against human CD20.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody has been used in western blot detection of CD20 in human plasma, revealing elevated CD20 levels in plasma from chronic lymphocytic leukemia patients (Manshouri et al, 2003). An scFv derived from this antibody fused to streptavidin has been investigated as a method of pretargeted radioimmunotherapy for lymphomas (Schultz et al, 2000), and has been demonstrated to be non-toxic in a phase I trial of non-Hodgkin lymphoma patients (Forero et al, 2004). Additionally, measles viruses engineered to express this scFv are able to enter CD20+ target cells through interaction with CD20 antigen, with clinical relevance for lymphoma therapy (Bucheit et al, 2003).

Comment:

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Background: MS4A1, B-lymphocyte antigen CD20,MS4A1, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, E9PPL6, P19437

UniProt: P11836