Recombinant CTNNA1 antibody

Catalog No. ABIN7543128

The Rabbit Chimeric anti-CTNNA1 antibody is suitable to detect CTNNA1 in samples from Human. It has been validated for WB, IF and IP.

Quick Overview for Recombinant CTNNA1 antibody (ABIN7543128)

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CTNNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 1G5

Product Details anti-CTNNA1 Antibody

Purpose: Anti-alpha catenin [1G5], Rabbit IgG, kappa

Specificity: This antibody recognizes alpha catenin and binds to the domain between amino acids 135-226. α-catenin associates with the cytoplasmic domain of a variety of plasma membrane spanning cadherins that mediate cell-cell adhesion. The association of catenins to cadherins produces a complex that is linked to the actin filament network, which seems to be of primary importance for their cell-adhesion properties.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing Balb/c mice with a 90 kDa MBP/alpha catenin fusion protein purified on an amylose column.

Isotype: IgG kappa

Application Details

Application Notes: This antibody is recommended for the detection and analysis of alpha-catenin via various methods. For instance, it was used to identify alpha-catenin in MDA468 cells via immunofluorescence (Bajpai et al., 2009). It was also repeatedly demonstrated that 1G5 specifically detects alpha-catenin on Western blots (Johnson et al., 1993, Stadler et al., 2018).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: alpha catenin

Background: Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, α-catenin, α catenin, α(E)-catenin

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location