Recombinant Desmoglein 3 antibody

Catalog No. ABIN7543134

The Rabbit Chimeric anti-Desmoglein 3 antibody has been validated for WB, IHC, IF, FACS, IP and DB. It is suitable to detect Desmoglein 3 in samples from Human.

Quick Overview for Recombinant Desmoglein 3 antibody (ABIN7543134)

Target: Desmoglein 3 (DSG3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Desmoglein 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Dot Blot (DB)

Clone: 5G11

Product Details anti-Desmoglein 3 Antibody

Purpose: Anti-desmoglein-3 [5G11], Rabbit IgG, kappa

Specificity: This antibody recognizes desmoglein 3. It does not cross-react with desmoglein 1 or desmoglein 2. Desmoglein-3 is a calcium binding membrane protein that is localized in desmosome cellular junctions and interacts with plaque proteins and intermediate filaments at cell-cell adhesion points.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing Balb/c mice with a recombinant protein corresponding to human desmoglein-3.

Isotype: IgG kappa

Application Details

Application Notes: This antibody can be used for detection and analysis of desmoglein-3 via various methods. For instance, 5G11 was utilized todetect desmoglein-3 in human scalp hair follicles and epidermal inclusions via immunohistochemistry (Wu et al., 2003). Another group successfully used this antibody to identify desmoglein-3 via Western blot (Mao et al., 2017, pmid: 28469076). The antibody 5G11 was also used to detect DSG3 in cells via immunofluorescence (Chen et al., 2013, pmid: 23737966).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Desmoglein 3

Target: Desmoglein 3 (DSG3)

Alternative Name: desmoglein-3

Background: PVA, Cadherin family member 6, desmoglein 3, 130 kDa pemphigus vulgaris antigen, DSG3

UniProt: P32926