Recombinant N-Cadherin antibody (Extracellular Domain)

Catalog No. ABIN7543136

This Rabbit Chimeric antibody specifically detects N-Cadherin in WB, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant N-Cadherin antibody (Extracellular Domain) (ABIN7543136)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 8C11

Product Details anti-N-Cadherin Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-N-cadherin [8C11], Rabbit IgG, kappa

Specificity: This antibody recognizes human, rabbit and hamster extracellular domain of N-cadherin. It does not cross react with this protein in mouse, rat cow and pig. N-cadherin is a calcium-dependent cell adhesion protein.

Cross-Reactivity: Hamster, Rabbit

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with an extracellular domain of the recombinant human N-cadherin.

Isotype: IgG kappa

Application Details

Application Notes: 8C11 antibody inhibits cells' motility, as it blocks extracellular domain of N-cadherin which is responsible for this function (Kim et al., 2000). This antibody has also been widely used in the detection and analysis of N-cadherin. For instance, it was utilized to identify this protein in A549 cells via immunofluorescence (Shintani et al., 2011). Another group used successfully 8C11 to analyze the levels of N-cadherin expression via Western blot (Chen et al., 2015). Finally, this antibody was used to label N-cadherin in tumor tissues of a metastasis model via immunohistochemistry (Li et al., 2019).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: N-cadherin

Background: CD325, Cadherin-2, CDw325, Neural cadherin, n-cadherin, ncadherin

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane