Recombinant IL2 Receptor beta antibody

Catalog No. ABIN7543149

The Mouse Monoclonal anti-IL2 Receptor beta antibody is suitable to detect IL2 Receptor beta in samples from Human. It has been validated for FACS, ELISA, IP, BR and InhA.

Quick Overview for Recombinant IL2 Receptor beta antibody (ABIN7543149)

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL2 Receptor beta antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: Mik-beta 1

Product Details anti-IL2 Receptor beta Antibody

Purpose: Anti-IL-2R Beta chain [Mik-beta 1], Mouse IgG2a, kappa

Specificity: This antibody recognizes and binds the beta chain of the IL-2 receptor and completely inhibits the IL-2 binding to the beta chain. The beta subunit is involved in receptor mediated endocytosis and transduction of the mitogenic signals of IL2.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BLAB/c mice with YTS cells, a human NK-like cell line that highly expresses the human IL-2R beta chain as immunogen.

Isotype: IgG2a kappa

Application Details

Application Notes: Mik-beta 1 inhibited binding of iodine labelled IL-2 to YTS cells in a dose dependent manner. Mik-beta 1 also successfully immunoprecipitated beta chain of the IL-2R from radio iodinated YTS extract. Biotinylated Mik-beta 1 also efficiently competes with IL-2 for binding to solubilized beta chain. Flow cytometry was used to study the cellular disctribution of beta chain, as determined by mik-beta 1 staining. This antibody completely inhibited IL-2 dependent T cell growth in presence of anti-Tac. Two-color flow cytometric analysis using anti-beta chain mAbs clearly showed that approximately 10 % of peripheral blood lymphocytes manifest the beta chain and that this population is mainly composed of Leu-19+ NK cells (PMID: 2467293). Mik beta 1 antibody acts synergistically with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells (PMID: 8335891). A recombinant single chain immunotoxin of Mik-beta 1 and Pseudomonas derived exotoxin PE40 was selectively cytotoxic for cells expressing high levels of IL-2R beta (p75) subunit (PMID: 1401913).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: IL-2R Beta chain

Background: CD122, IL-2R, IL2Rβ, IL-2RB, p70-75, p75, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, Mik-β1

UniProt: P14784

Pathways: JAK-STAT Signaling, Growth Factor Binding