Recombinant IL2 Receptor beta antibody

Catalog No. ABIN7543150

This anti-IL2 Receptor beta antibody is a Rabbit Chimeric antibody detecting IL2 Receptor beta in FACS, ELISA, IP, BR and InhA. Suitable for Human.

Quick Overview for Recombinant IL2 Receptor beta antibody (ABIN7543150)

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This IL2 Receptor beta antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: Mik-beta 1

Product Details anti-IL2 Receptor beta Antibody

Purpose: Anti-IL-2R Beta chain [Mik-beta 1], Rabbit IgG, kappa

Specificity: This antibody recognizes and binds the beta chain of the IL-2 receptor and completely inhibits the IL-2 binding to the beta chain. The beta subunit is involved in receptor mediated endocytosis and transduction of the mitogenic signals of IL2.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BLAB/c mice with YTS cells, a human NK-like cell line that highly expresses the human IL-2R beta chain as immunogen.

Isotype: IgG kappa

Application Details

Application Notes: Mik-beta 1 inhibited binding of iodine labelled IL-2 to YTS cells in a dose dependent manner. Mik-beta 1 also successfully immunoprecipitated beta chain of the IL-2R from radio iodinated YTS extract. Biotinylated Mik-beta 1 also efficiently competes with IL-2 for binding to solubilized beta chain. Flow cytometry was used to study the cellular disctribution of beta chain, as determined by mik-beta 1 staining. This antibody completely inhibited IL-2 dependent T cell growth in presence of anti-Tac. Two-color flow cytometric analysis using anti-beta chain mAbs clearly showed that approximately 10 % of peripheral blood lymphocytes manifest the beta chain and that this population is mainly composed of Leu-19+ NK cells (PMID: 2467293). Mik beta 1 antibody acts synergistically with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells (PMID: 8335891). A recombinant single chain immunotoxin of Mik-beta 1 and Pseudomonas derived exotoxin PE40 was selectively cytotoxic for cells expressing high levels of IL-2R beta (p75) subunit (PMID: 1401913).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: IL-2R Beta chain

Background: CD122, IL-2R, IL2Rβ, IL-2RB, p70-75, p75, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, Mik-β1

UniProt: P14784

Pathways: JAK-STAT Signaling, Growth Factor Binding