Recombinant Mesothelin antibody

Catalog No. ABIN7543155

The Mouse Monoclonal anti-Mesothelin antibody has been validated for ELISA, WB, IHC, IF, SPR and RIA. It is suitable to detect Mesothelin in samples from Human.

Quick Overview for Recombinant Mesothelin antibody (ABIN7543155)

Target: Mesothelin (MSLN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Surface Plasmon Resonance (SPR), Radioimmunoassay (RIA)

Clone: K1

Product Details anti-Mesothelin Antibody

Purpose: Anti-Mesothelin [K1], Mouse IgG1, kappa

Specificity: This antibody can bind the human mesothelium, expressed on many cancer cells like ovarian carcinoma.

Cross-Reactivity: Cynomolgus

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunization of mice with periodate-treated human ovarian cancinoma (OVCAR-3) cells. These mice had previously been made tolerant to normal human kidney membranes.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody was used for the study and characterization of CAK1 antigen present on ovarian cancers and normal mesothelium. The initial screening of the mesothelin reactive clones to live OVCAR-3 cells was done using immunofluorescence. Secondary screening was done using immunoperoxidase histochemistry on cryostat sections of normal human tissues and human tumors. Quantitative ELISA confirmed that K1 and OC125 antibodies bind to different epitopes on the OVCAR-3 cells (PMID: 1735605). This antibody can detect mesothelin in a western blot assay. Competition RIA was used to detect the presence of CAK1 antigen in the supernatent of protease or phosphatidylinositol-phospholipase C treated cells. An immunotoxin composed of K1 antibody and native Pseudomonas exotoxin was found to be selectively cytotoxic to cells expressing CAK1 antigen (PMID: 1727378). The affinity of K1 antibody to mesothelin was Kd= 8 nM and determined using surface plasmon resonance (BIAcore assay) (PMID: 9178820).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: Mesothelin

Background: MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process