Recombinant Disialoganglioside 2 (GD2) antibody

Catalog No. ABIN7543163

This Rabbit Chimeric antibody specifically detects Disialoganglioside 2 in FACS and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant Disialoganglioside 2 (GD2) antibody (ABIN7543163)

Target: Disialoganglioside 2 (GD2)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), ELISA

Clone: 14G2a

Product Details

Purpose: Anti-Disialoganglioside 2 [14G2a], Rabbit IgG, kappa

Specificity: This antibody binds the GD2 antigen and GD2 expressing neuroblastoma cell lines with an affinity similar to the parent antibody.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original murine parent antibody was generated against human neuroblastoma cell line LAN-1. This IgG2a isotype version was then generated by class switching of the murine parent antibody 14.18 (IgG3).

Isotype: IgG kappa

Application Details

Application Notes: This antibody is a IgG2a isotype switch variant of the murine parent antibody 14.18. This antibody can direct efficient complement-dependent cytotoxicity against neuroblastoma tumor cells and melanoma cell lines in vitro in the presence of human complement (PMID: 2720646). 14G2a was coupled to the ribosome-inactivating plant toxin gelonin with the heterobifunctional cross-linking reagentN-succinimidyl-3(2-pyridyldithio)propionate. The immunoreactivity and specificity of 14G2a-gelonin immunotoxin determined by ELISA. Assays for complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC) revealed that these functional properties of the native 14G2a antibody were also preserved in the 14G2a-gelonin immunotoxin (PMID: 1756537). An immunotoxin prepared by linked 14G2a via the bivalent linker N-succinimidyloxycarbonyl-amethyl-a-(2-piridyldithio)toluene to deglycosylated ricin A chain was shown to inhibit the protein synthesis of neuroblastoma cell lines IMRS and NMB (Gottstein et al., 1994). Treatment with 14G2a mAb (1-4 mg/kg cumulative dose given as five i.v. daily administrations) dramatically reduced the metastatic spread of NB cells and prolonged the long-term survival of treated mice in a dose-dependent manner (PMID: 12880983). This antibody is also reported to be used in the study of interactions between peptides mimicking the GD2 ganglioside using flow ctyometry and ELISA (PMID: 21455557).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Disialoganglioside 2 (GD2)

Alternative Name: Disialoganglioside 2

Background: GD2