Recombinant TPBG antibody (Extracellular Domain)

Catalog No. ABIN7543174

The Mouse Monoclonal anti-TPBG antibody (Clone 5T4) (ABIN7543174) specifically detects TPBG in ELISA, IHC, IP, IF, DB and RIA. The antibody is reactive with Human samples.

Quick Overview for Recombinant TPBG antibody (Extracellular Domain) (ABIN7543174)

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TPBG antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Radioimmunoassay (RIA)

Clone: 5T4

Product Details anti-TPBG Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-5T4 [5T4 (H8)], Mouse IgG1, kappa

Specificity:

This antibody binds 5T4 antigen expressed on various carcinomas. It is reported that epitope recognized by this antibody is present in the extracellular domain of the molecule more particularly towards the membrane proximal leucine rich repeat 2 (LRR2) (PMID: 11903056).

Application Notes original Clone: This antibody binds 5T4 antigen with a very high affinity of K(D)=1.8x10(-11) M (PMID: 11113573). The reactivity of this antibody to solubilised human syncytiotrophoblast plasma membrane (StMPM) was checked using immunodotting on nitrocellulose membrane and ELISA. The distribution of the 5T4 antigen was determined using mAb 5T4 by indirect immunoperoxidase staining of sections of normal organ and placental tissues as well as immunofluorescence and radiobinding assays with a wide variety of cell lines representing differing normal and tumour cell types. Immunoprecipitation of radiolabelled StMPM using this antibody indicated that 5T4 Molecules are glycoproteins of mol. wt of approximately 72 kD on SDS-PAGE (PMID: 3355761). This paper (PMID: 2404511) studies the immunohistochemical distribution of 5T4 antigen and reports that 54T4 antigen is present in a wide range of tumor cells including carcinomas of the bladder, breast, cervix, endometrium, lung, oesophagus, ovary, pancreas, stomach and testicular non-seminomatous germ cell tumours. 5T4 can serve as a marker for detection of tumor. Various papers report the use of this antibody for the study of expression of 5T4 antigen in various types of cancers (PMID: 2404512, 1419629, 8180020, 11578488).

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing male BALB/c mice with wheat germ agglutinin (WGA) purified glycoproteins from deoxycholate (DOC) solubilised human syncytiotrophoblast plasma membrane (StMPM).

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TPBG

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Alternative Name: 5T4

Background: TPBG, M6P1, WAIF1, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1

UniProt: Q13641