Recombinant HDGF antibody

Catalog No. ABIN7543194

The Rabbit Chimeric anti-HDGF antibody has been validated for WB, IHC, IF, IP and Neut. It is suitable to detect HDGF in samples from Human.

Quick Overview for Recombinant HDGF antibody (ABIN7543194)

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This HDGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Neutralization (Neut)

Clone: H3

Product Details anti-HDGF Antibody

Purpose: Anti-HDGF [H3], Rabbit IgG, kappa

Specificity: This antibody is specific for the HDGF protein.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was raised by immunizing mice with full-length recombinant HDGF. This antibody binds a different epitope on HDGF than antibody C1.

Isotype: IgG kappa

Application Details

Application Notes: This antibody was used for immunoprecipitation on total cell lysates from H460 NSCLC cells (US20070243191). The neutralizing ability of this antibody was tested on A549. The result was a significant decrease in tumor size (US20070243191). This antibody was fluorescently labelled and injected into nude mice to test the antibody distribution. The antibodies evenly distributed in the animals including the tumors. This antibody entered into the cytoplasmic compartment of some tumor cells (US20070243191). Immunohistochemistry with this antibody was performed on tissues from NSCLC patients (US20070243191). Western blot was performed on HDGF protein using this antibody (Ren et al, 2009,pmid:19435872). ELISA was performed on the HDGF protein using this antibody (US20070243191).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for HDGF

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Alternative Name: HDGF

Background: Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

UniProt: P51858

Pathways: ER-Nucleus Signaling