Recombinant Desmoglein 3 antibody

Catalog No. ABIN7543212

The Rabbit Chimeric anti-Desmoglein 3 antibody has been validated for ELISA, IF and IP. It is suitable to detect Desmoglein 3 in samples from Human.

Quick Overview for Recombinant Desmoglein 3 antibody (ABIN7543212)

Target: Desmoglein 3 (DSG3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Desmoglein 3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AK23

Product Details anti-Desmoglein 3 Antibody

Purpose: Anti-Desmoglein 3 [AK23], Rabbit IgG, kappa

Specificity: This antibody binds mouse Dsg3 and also shows reactivity for human Dsg3. It does not show any affinity towards Dsg1. AK23 recognizes a calcium-dependent conformational epitope on Dsg3, which consisted of the V3, K7, P8, and D59 Dsg3-specific residues that formed the adhesive interface between juxtaposed Dsg.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Dsg3 knockout mice with recombinant mouse Dsg3-His in complete Freund's adjuvant subcutaneously.

Isotype: IgG kappa

Application Details

Application Notes: This antibody can be used to study the blister formation and complex interaction that occur in autoimmune blistering disease like Pemphigus vulgaris (PV). Pemphigus vulgaris (PV) is an epidermal blistering disorder caused by antibodies directed against the desmosomal cadherin desmoglein-3 (Dsg3). AK23 was reported to induce pemphigus vulgaris (PV) blisters in neonatal mice. The binding affinity of this antibody for human and mouse desmoglein 3 was determined using ELISA. This antibody was also used in the live keratinocyte staining of mouse PAM212 cell line and indirect immunofluorescent staining of mouse oral mucuous membrance, mouse liver and lower parts of the human epidermis. Immunoprecipitaton was used for the determination of the epitope of the antibody (PMID: 12574390). This antibody was also used in the study of alteration in desmosome morphology (PMID: 23226536).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Desmoglein 3

Target: Desmoglein 3 (DSG3)

Alternative Name: Desmoglein 3

Background: DSG3, PVA, CDHF6, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6

UniProt: P32926