Recombinant Liver Arginase antibody

Catalog No. ABIN7543254

The Rabbit Chimeric anti-Liver Arginase antibody has been validated for cryo-EM and Inh. It is suitable to detect Liver Arginase in samples from Human.

Quick Overview for Recombinant Liver Arginase antibody (ABIN7543254)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Cryogenic electron microscopy (cryo-EM), Inhibition (Inh)

Clone: MAb1

Product Details anti-Liver Arginase Antibody

Purpose: Anti-Arginase 1 [mAb1], Rabbit IgG, kappa

Specificity: The antibody sterically blocks the arginase 1 active site near the exterior surface of the enzyme. Approximately 80 % of all complexes identified were composed of two arginase 1 trimers and three antibodies, forming a 2:3 complex, while the remaining 20 % of complexes were composed of two arginase 1 trimers and two antibodies, however, the epitope and paratope interactions between each antibody and arginase 1 monomers are identical. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The antibody is a human antibody identified via yeast display technologies and isolated after panning against arginase 1.

Isotype: IgG kappa

Application Details

Application Notes: The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of human arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki for the antibody against human Arg1 was 3.3 ± 0.3 nM (Palte et al., 2021, PMID: 34326456).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: Arginase 1

Background: ARG1, Arginase-1, Liver-type arginase, Type I arginase

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin