Recombinant TREM1 antibody

Catalog No. ABIN7543259

This Mouse Monoclonal antibody specifically detects TREM1 in FACS, ELISA and BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant TREM1 antibody (ABIN7543259)

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TREM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: 14F69

Product Details anti-TREM1 Antibody

Purpose: Anti-TREM-1 [14F69], Mouse IgG2a, kappa

Specificity: This antibody binds human, cynomolgus monkey and rhesus monkey TREM-1. TREM1 is a receptor that is expressed on monocytes, macrophages, neutrophils and dendritic cells. It plays important roles in innate and adaptive immunity by amplifying inflammatory responses. When activated, TREM-1 associates with the ITAM-containing signaling adaptor protein, DAP12. Downstream signalling may include activation of the NFAT transcription factor, causing an upregulation of pro-inflammatory cytokine production.

Cross-Reactivity: Cynomolgus, Rhesus Monkey

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both.

Isotype: IgG2a kappa

Application Details

Application Notes: The original IgG2a antibody binds human TREM-1 with an affinity of 2 X 10^-10 nM as tested by suface plasmon resonance. The screening of original antibody for binding human TREM-1 was done using ELISA and flow cytometry. This antibody is capable of binding both human, cynomolgus monkey and rhesus monkey PBMCs. This antibody was reported to block the release of TNFalpha from stimulated peripheral blood mononuclear cells (PBMCs). This antibody was also able to block the ligand-induced TREM-1 activation in such BWZ/hTREM-1 reporter cell assay (US9000127).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TREM1

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Alternative Name: TREM-1

Background: TREM1, CD354, Triggering receptor expressed on myeloid cells 1, Triggering receptor expressed on monocytes 1

UniProt: Q9NP99