Recombinant p40 (p63 Delta) (AA 4-19) antibody

Catalog No. ABIN7543298

Product Details

Target: p40 (p63 Delta)

Antibody Type: Recombinant Antibody

Binding Specificity: AA 4-19

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purpose: Anti-p40 [14D5E8F12], Rabbit IgG, kappa

Specificity: This antibody is specific for the p40 peptide of the human p63. P63 protein acts as a sequence specific DNA binding transcriptional activator or repressor. It is also involved in notch signaling by probably inducing JAG1 and JAG2 and plays a role in the regulation of epithelial morphogenesis. The p63 Molecule has a high degree of structural homology with p53 and has three similar functional domains: N-terminal transcription activation region, core DNA binding region, and C-terminal oligomerization region.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with p40 peptide comprising amino acids 4-19 (LENNAQTQFSEPQYTN) of the p63 protein (Uniprot -Q9H3D4). The p40 peptide had a sulfhydryl group at its N-terminus and a carrier protein KLH to improve its immunogenicity.

Clone: 14D5E8F12

Isotype: IgG kappa

Application Details

Application Notes: This antibody is highly similar to commercially available rabbit antibody ZR8 in immunohistochemical staining of p40. This antibody does not stain lung adenocarcinoma but positively stains lung squamous cell carcinoma. This antibody can used for the differential diagnosis of lung squamous cell carcinoma and lung adenocarcinoma via immunohistochemical staining. This binding specificity and affinity of this antibody was determined using ELISA. The original IgG1 antibody is reported to bind human p40 with an affinity constant of 5.99 X 10^9 M^(-1) (CN104447994).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: p40 (p63 Delta)

Alternative Name: p40

Background: TP63, CUSP, Tumor protein 63, chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p73L, p40, p51, ZR8

UniProt: Q9H3D4