Recombinant KRT14 antibody (AA 458-472)

Catalog No. ABIN7543316

This Rabbit Chimeric antibody specifically detects KRT14 in IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant KRT14 antibody (AA 458-472) (ABIN7543316)

Target: KRT14 (Keratin 14 (KRT14))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This KRT14 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: 2G4

Product Details anti-KRT14 Antibody

Binding Specificity: AA 458-472

Purpose: Anti-Cytokeratin 14 [2G4], Rabbit IgG, kappa

Specificity: This antibody is capable of binding human cytokeratin 14. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 458-472 of cytokeratin 7 in equal volume of Freund's incomplete adjuvant.

Isotype: IgG kappa

Application Details

Application Notes: The binding characterization of this antibody was done using ELISA. The original IgG1 antibody binds cytokeratin 5/6 with a high affinity constant of 4.8x 10^(8) M^(-1). This antibody was also used in the immunohistochemical analysis of frozen paraffin sections of tonsil tissues and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody L1002 (CN113087793).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14

Background: CK14, CK-14, CK 14, K14, KRT14, keratin type I cytoskeletal 14, cytokeratin-14, keratin-14, CGMCC NO 20761, L1002

UniProt: P02533