Recombinant MSH6 antibody

Catalog No. ABIN7543329

The Rabbit Chimeric anti-MSH6 antibody has been validated for WB, IHC and ELISA. It is suitable to detect MSH6 in samples from Human.

Quick Overview for Recombinant MSH6 antibody (ABIN7543329)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This MSH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: ABM58060-20A2-PU

Product Details anti-MSH6 Antibody

Purpose: Anti-MSH6 [ABM58060-20A2-PU], Rabbit IgG, kappa

Specificity: This antibody binds human DNA mismatch repair protein MSH6. MSH6 forms a heterodimer with MSH2 to make the human MutSα mismatch repair complex and initiates DNA repair. The DNA mismatch repair system (MMR) is composed of enzyme molecules that specifically repair DNA base mismatches. Defects in expression of MSH6 are often associated with hereditary non-polypatopathic colorectal cancer (HNPCC).

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant human MSH6 protein.

Isotype: IgG kappa

Application Details

Application Notes: The binding specificity of this antibody for human MSH6 was determined using ELISA. This antibody was capable of detecting recombinant MSH6 in a western blot. This antibody was also used in the immunohistochemical analysis of colon cancer, bladder cancer and testicular tissues. This antibody shows similar staining of MSH6 protein compared to the commercially available antibody 44 (CN111454365).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6

Background: HMSH6, p160, GTBP, GTMBP, DNA mismatch repair protein Msh6, G/T mismatch-binding protein, MutS protein homolog 6, MutS-alpha 160 kDa subunitC, GMCC NO.17492, 44

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response