Recombinant Histone H4 antibody
Quick Overview for Recombinant Histone H4 antibody (ABIN7543353)
Target
See all Histone H4 (HISTH4) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Anti-Histone H4 [1A9D7], Mouse IgG1, kappa
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Specificity
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The antibody specifically recognizes simultaneous acetylations at K5 and K8 of histone H4. Lysine acetylation of core histones is one of the major posttranslational modifications involved in the control of eukaryotic gene expression.
Application Notes original Clone: The specificity of the original format of the antibody for histone H4 proteins containing di-acetylation of K5acK8ac was confirmed by ELISA analysis. The antibody detected the histone H4 proteins containing at least the di-acetylation of K5acK8ac western blot analysis. The crystal structure of the complex of di-acetylated histone H4 and 1A9D7 Fab fragment was determined (Handoko et al., 2018, PMID: 30080437).
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Characteristics
- This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format for improved compatibility with existing reagents assays and techniques.
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Purification
- Protein A affinity purified
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Immunogen
- The original antibody was generated by immunizing mice with Histone H4 protein with site-specific acetylations at K5 and K8.
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Isotype
- IgG1, kappa
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anti-Histone H4 (HISTH4) (H4K12ac) antibody
HISTH4 Reactivity: Human, Mouse, Rat, Monkey WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Histone H4 (HISTH4) (2meLys20) antibodyHISTH4 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-Histone H4 (HISTH4) (3meLys20) antibodyHISTH4 Reactivity: Human WB, IHC, IF, DB Host: Rabbit Polyclonal unconjugated
anti-Histone H4 (HISTH4) (acLys8) antibodyHISTH4 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-Histone H4 (HISTH4) (acLys5) antibodyHISTH4 Reactivity: Human WB, IHC, IF Host: Rabbit Monoclonal unconjugated
anti-Histone H4 (HISTH4) (AA 2-103) antibodyHISTH4 Reactivity: Human, Mouse, Rat, Pig, Cow, Chicken, Horse, Guinea Pig, Dog, Cat, Rhesus Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Histone H4 (HISTH4) (H4K16ac) antibodyHISTH4 Reactivity: Human, Mouse, Saccharomyces cerevisiae, Drosophila melanogaster WB, IF, ChIP, DB, ChIP-seq, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone H4 (HISTH4) (acLys8) antibodyHISTH4 Reactivity: Human, Mouse WB, IF, ICC, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-Histone H4 (HISTH4) (acLys5) antibodyHISTH4 Reactivity: Human, Mouse WB, IF, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-Histone H4 (HISTH4) (meArg3) antibodyHISTH4 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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OriginalSpeciesName: Mouse
OriginalFormat: IgG1
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- PBS with 0.02 % Proclin 300.
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Histone H4 (HISTH4)
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Alternative Name
- Histone H4
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Background
- H4K5acK8ac, Histone H4 protein,
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UniProt
- P62805
Target
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