Recombinant CDX2 antibody

Catalog No. ABIN7543367

This Rabbit Chimeric antibody specifically detects CDX2 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CDX2 antibody (ABIN7543367)

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CDX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 12B6

Product Details anti-CDX2 Antibody

Purpose: Anti-CDX2 [12B6], Rabbit IgG, kappa

Specificity: The antibody recognises human CDX2. The CDX2 protein is a homeobox transcription factor expressed in the nuclei of intestinal epithelial cells, playing an essential role in the development and function of the digestive system.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing a mouse with the CDX2-KLH protein.

Isotype: IgG kappa

Application Details

Application Notes: The antibody was used for immunohistochemistry on stomach adenocarcinoma, showing accurate staining location, clear staining, no non-specific staining, and clean background. Further, immunohistochemistry staining on stomach adenocarcinoma from 12B6 antibody and the commercial antibody, rabbit mono-ERP2764Y, were compared. Results showed the staining intensity of 12B6 antibody was significantly higher than those of ERP2764Y, showing 12B6 antibody is more sensitive. Moreover, the antibody and the commercial antibody TA347 were used for immunohistochemistry on appendix tissue. Results showed that the antibody (12B6) and the commercially available antibody (TA347) were simultaneously detected on the normal tissue chip, and the negative and positive detection results were consistent, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody. The antibody detected human CDX2 by western blot analysis (CN113265000).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CDX2

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Alternative Name: CDX2

Background: Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2, rabbit mono-ERP2764Y, TA347

UniProt: Q99626

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance