Recombinant TLR7 antibody
Quick Overview for Recombinant TLR7 antibody (ABIN7543379)
Target
See all TLR7 AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Anti-TLR7 [LTM3], Mouse IgG1, kappa
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Specificity
- This antibody binds human toll-like receptor 7 (TLR7). It also cross reacts with monkey TLR7. It is an endosomal receptor that plays a key role in innate and adaptive immunity. It also controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. TLR7 is considered to be a useful marker in indicating pathological condition of autoimmune diseases.
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Cross-Reactivity
- Monkey
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Characteristics
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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Purification
- Protein A affinity purified
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Immunogen
- The original antibody was generated by immunizing TLR7 deficient BALB/mice with Ba/F3 cell line expressing hTLR7-Flag-Hisx6/hUnc93B1-HAx2 mixed with a suitable adjuvant.
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Isotype
- IgG1 kappa
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Application Notes
- This antibody was used in combination with another anti-TLR7 antibody called rE3 to detect human TLR7 in a sandwich ELISA. LTM3 was used as used as a capturing antibody and biotinylated rE3 antibody (rE3-bio) was used a detecting antibody. This antibody can be used for the detection of human TLR7 in serum or plasma derived from human peripheral blood. The binding ability of this antibody for Ba/F3 cell line expressing human TLR7, mouse TLR7 and monkey TLR7 was checked using flow cytometry. This antibody can successfully detect human and monkey TLR7, but not mouse TLR7 in a flow cytometry (US20210199667).
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- PBS with 0.02 % Proclin 300.
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- TLR7 (Toll-Like Receptor 7 (TLR7))
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Alternative Name
- TLR7
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Background
- Toll-like receptor 7, UNQ248/PRO285
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UniProt
- Q9NYK1
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Pathways
- TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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