Recombinant TLR7 antibody

Catalog No. ABIN7543379

This Mouse Monoclonal antibody specifically detects TLR7 in ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant TLR7 antibody (ABIN7543379)

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR7 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: LTM3

Product Details anti-TLR7 Antibody

Purpose: Anti-TLR7 [LTM3], Mouse IgG1, kappa

Specificity: This antibody binds human toll-like receptor 7 (TLR7). It also cross reacts with monkey TLR7. It is an endosomal receptor that plays a key role in innate and adaptive immunity. It also controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. TLR7 is considered to be a useful marker in indicating pathological condition of autoimmune diseases.

Cross-Reactivity: Monkey

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing TLR7 deficient BALB/mice with Ba/F3 cell line expressing hTLR7-Flag-Hisx6/hUnc93B1-HAx2 mixed with a suitable adjuvant.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody was used in combination with another anti-TLR7 antibody called rE3 to detect human TLR7 in a sandwich ELISA. LTM3 was used as used as a capturing antibody and biotinylated rE3 antibody (rE3-bio) was used a detecting antibody. This antibody can be used for the detection of human TLR7 in serum or plasma derived from human peripheral blood. The binding ability of this antibody for Ba/F3 cell line expressing human TLR7, mouse TLR7 and monkey TLR7 was checked using flow cytometry. This antibody can successfully detect human and monkey TLR7, but not mouse TLR7 in a flow cytometry (US20210199667).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TLR7

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Alternative Name: TLR7

Background: Toll-like receptor 7, UNQ248/PRO285

UniProt: Q9NYK1

Pathways: TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades