Recombinant Laminin antibody

Catalog No. ABIN7543381

The Human Monoclonal anti-Laminin antibody (Clone C3_Hu10) (ABIN7543381) specifically detects Laminin in ELISA, WB, IF and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant Laminin antibody (ABIN7543381)

Target: Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Human

Clonality: Monoclonal

Conjugate: This Laminin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: C3_Hu10

Product Details anti-Laminin Antibody

Purpose: Anti-Laminin-2 LG5 [C3_Hu10], Human IgG1, kappa

Specificity:

This antibody recognizes human and mouse laminin-2 LG5 and LG4/5 domain. Laminin is a large extracellular heterotrimeric glycoprotein and a major constituent of the basement membrane. It is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Application Notes original Clone: The binding specificity of this antibody for human and mouse laminin-2 LG5 was confirmed using ELISA. This antibody is capable of detecting human and mouse LG-4/5 expressed on HEK293 in a flow cytometry. This antibody can also be used for the identification recombinant human laminin-2 (Hu 211), murine LG-5 (mLG5), human LG-5 (hLG5), and human LG-4/5 (hLG4/LG5) in a western blot. This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. This antibody was used in combination with anti-laminin-2 antibody AS30_Hu6 to generate a duobody called TBTI (US11254737).

Cross-Reactivity: Mouse

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Balb/c and Trianni mice with human laminin-2 LG5 domain. Humanized version was generated by grafting CDRs of parental mouse antibody C3 onto human framework regions.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Human

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Laminin

Target: Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))

Alternative Name: Laminin-2 LG5

Background: Merosin, Laminin subunit alpha-2, LAMA2, Laminin 211, laminin G-like 5 domain, LG5, g-like 4 and 5 domain, LG-4/5, Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain, C3, humanized C3

UniProt: P24043, Q60675