Recombinant Laminin antibody

Catalog No. ABIN7543382

This Rabbit Chimeric antibody specifically detects Laminin in ELISA, WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Laminin antibody (ABIN7543382)

Target: Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Laminin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: C3_Hu10

Product Details anti-Laminin Antibody

Purpose: Anti-Laminin-2 LG5 [C3_Hu10], Rabbit IgG, kappa

Specificity: This antibody recognizes human and mouse laminin-2 LG5 and LG4/5 domain. Laminin is a large extracellular heterotrimeric glycoprotein and a major constituent of the basement membrane. It is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Balb/c and Trianni mice with human laminin-2 LG5 domain. Humanized version was generated by grafting CDRs of parental mouse antibody C3 onto human framework regions.

Isotype: IgG kappa

Application Details

Application Notes: The binding specificity of this antibody for human and mouse laminin-2 LG5 was confirmed using ELISA. This antibody is capable of detecting human and mouse LG-4/5 expressed on HEK293 in a flow cytometry. This antibody can also be used for the identification recombinant human laminin-2 (Hu 211), murine LG-5 (mLG5), human LG-5 (hLG5), and human LG-4/5 (hLG4/LG5) in a western blot. This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. This antibody was used in combination with anti-laminin-2 antibody AS30_Hu6 to generate a duobody called TBTI (US11254737).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Laminin

Target: Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))

Alternative Name: Laminin-2 LG5

Background: Merosin, Laminin subunit alpha-2, LAMA2, Laminin 211, laminin G-like 5 domain, LG5, g-like 4 and 5 domain, LG-4/5, Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain, C3, humanized C3

UniProt: P24043, Q60675