Recombinant ANO1 antibody

Catalog No. ABIN7543397

This Rabbit Chimeric antibody specifically detects ANO1 in IHC, WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant ANO1 antibody (ABIN7543397)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: 19F2

Product Details anti-ANO1 Antibody

Purpose: Anti-DOG1 [19F2], Rabbit IgG, kappa

Specificity: The antibody is specific for DOG1. DOG1 is a calcium-activated chloride channel (CaCC) which plays a role in the transepithelial anion transport and smooth muscle contraction. It is required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles and it acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with recombinant protein DOG1.

Isotype: IgG kappa

Application Details

Application Notes: The antibody 19F2 and the commercial antibody SP31 were used to stain breast tissue and gastrointestinal tumor in immunohistochemistry. Results showed the antibody specificity is equivalent to the commercial antibody. Further, the staining intensity of the antibody 19F2 was higher than the commercial antibody (CN113045652A).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: DOG1

Background: Discovered On Gastrointestinal Stromal Tumors Protein 1, SP31, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2, CGMCC NO. 20771

UniProt: Q5XXA6