Recombinant LAG3 antibody

Catalog No. ABIN7543410

The Rabbit Chimeric anti-LAG3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect LAG3 in samples from Human.

Quick Overview for Recombinant LAG3 antibody (ABIN7543410)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This LAG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 22G3

Product Details anti-LAG3 Antibody

Purpose: Anti-LAG-3 [22G3], Rabbit IgG, kappa

Specificity: The antibody is specific for LAG3. Lymphocyte activation gene 3 protein is an inhibitory receptor on antigen activated T-cells. It delivers inhibitory signals upon binding to ligands, such as FGL1.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with recombinant LAG3 protein.

Isotype: IgG kappa

Application Details

Application Notes: The antibody specifically detected LAG3 protein in Western blot analysis. The antibody and the commercial antibody EPR20261 were used to stain tonsillar granulocytes and granulocytes in the appendix. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 22G3 was higher than the commercial antibody. The antibody and the commercially available antibody E3 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113234159A).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG-3

Background: CD223, LAG3, Lymphocyte-activation gene-3, Lymphocyte activation gene 3 protein, LAG-3, CGMCNO 20774

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints