Recombinant IL-18 antibody

Catalog No. ABIN7543422

The Rabbit Chimeric anti-IL-18 antibody has been validated for IP, ELISA, FACS and Neut. It is suitable to detect IL-18 in samples from Human.

Quick Overview for Recombinant IL-18 antibody (ABIN7543422)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This IL-18 antibody is un-conjugated

Application: Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Clone: ABT-325 )

Product Details anti-IL-18 Antibody

Purpose: Anti-IL-18 [ABT-325 (A-794722.0, 2.5(E))], Rabbit IgG, kappa

Specificity: This antibody binds human and cynomolgus monkey IL-18 and does not cross react with mouse, rat or dog IL-18. Interleukin-18 is a pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses.

Cross-Reactivity: Cynomolgus

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing transgenic Xenomouse with human IL-18 in phosphate-buffered saline.

Isotype: IgG kappa

Application Details

Application Notes: The binding characterization of this antibody to human and cynomolgus monkey IL-18 was done using BIACORE assay. The binding affinity of the human IgG1 version was reported to be Kd=0.196 nM. This antibody can be used to immunoprecipitate human and cynomolgus monkey IL-18. This antibody neutralized both recombinant (KG-1 and RBA) and natural IL-18 (WBA) in an in vitro assay. The ability of this antibody to neutralize natural human IL-18-induced IFNγ in an inflammatory environment in vivo, the severe combined immunodeficient (SCID) mouse model was used wherein human PBMCs were injected into the mouse and the cells were stimulated in vivo to produce human IL-18 (HuPBMC-SCID model). This antibody inhibited human IL-18-dependent human IFNγ production in vivo with a clear dose-response by either route of administration. The binding of C1q to this antibody was determined using standard ELISA techniques (US20050100965). This antibody was used in the generation of a dual variable domain immunoglobulin (DVD-Ig) in combination with anti-IL-12 antibody for therapeutic use for targeting disease where multiple mediators contribute to overall disease pathogenesis (PMID: 17934452).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL-18

Background: Interleukin-18, IL18, IGIF, IL1F4, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, ABT325, 2.5(E)mg1

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome