Recombinant FTH1 antibody

Catalog No. ABIN7543446

The Mouse Monoclonal anti-FTH1 antibody is suitable to detect FTH1 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for Recombinant FTH1 antibody (ABIN7543446)

Target: FTH1 (Ferritin Heavy Chain 1 (FTH1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 4C9

Product Details anti-FTH1 Antibody

Purpose: Anti-Ferritin H [4C9], Mouse IgG1, kappa

Specificity: This antibody binds human acidic isoferritin. Ferritin is a protein that catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Ferritin is important for iron homeostasis. Ferritins occur as many isoforms, all of which are composed of various combinations of the two primary subunits, H and L. Acidic isoferritins have high proportions of H subunit.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with acidic isoferritin (AIF) from primary hepatic carcinoma (PHC) tissues.

Isotype: IgG1 kappa

Application Details

Application Notes: The binding specificity of this antibody towards acidic isoferritin, basic isoferritin and human liver ferritin was determined using ELISA. This antibody does not cross react with basic isoferritin and human liver ferritin. The binding affinity of the original mouse IgG1 format of this antibody for acidic isoferritin was reported to be Kd= 4.15 nM. This antibody was used for the immunohistochemical staining of liver tissues of primary hepatic carcinoma (PHC)(PMID: 11604110). This antibody can be used for the identification of acidic isoferritins in a western blot (PMID: 12697395).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for FTH1

Target: FTH1 (Ferritin Heavy Chain 1 (FTH1))

Alternative Name: Ferritin H

Background: FTH1, FTHL6, AIF, acidic isoferritin, Ferritin heavy chain, Ferritin H subunit

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis