Recombinant Iba1 antibody

Catalog No. ABIN7543454

This Rabbit Monoclonal antibody specifically detects Iba1 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Iba1 antibody (ABIN7543454)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: GT1-mAb1

Product Details anti-Iba1 Antibody

Purpose: Anti-AIF1 [GT1-mAb1], Rabbit IgG, lambda

Specificity: This antibody is specific to < a href="https://everestbiotech.com/product/ebp05419-immunizing-peptide/" target="_blank">EBP05419, an immunizing peptide with the sequence C-TGPPAKKAISELP. This peptide was used in the production of the < a href="https://everestbiotech.com/product/goat-anti-aif1-iba1-isoform-1-and-3-antibody/" target="_blank">polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.

Characteristics:

Original Species of Ab: Goat

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.

Isotype: IgG lambda

Application Details

Application Notes: This antibody (GT1-mAb1) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb1 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb1 was found to bind the target peptide in a non-competitive manner with GT1-mAb2 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb1 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb1 was compatible with immunofluorescence (IF), flow cytometry (FC), and Western blot (WB), with an FC signal observedly more distinct than that of GT1-mAb2.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, Daintain, DADB-70P7.8, allograft inflammatory factor-1 splice variant Hara-1, IRT1, protein G1

UniProt: P55008

Pathways: Smooth Muscle Cell Migration