Recombinant TCR gamma/delta antibody

Catalog No. ABIN7543458

The Mouse Monoclonal anti-TCR gamma/delta antibody is suitable to detect TCR gamma/delta in samples from Human. It has been validated for FACS and Func.

Quick Overview for Recombinant TCR gamma/delta antibody (ABIN7543458)

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR gamma/delta antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: G5-4

Product Details anti-TCR gamma/delta Antibody

Purpose: Anti-Gamma Delta TCR [G5-4], Mouse IgG2a, kappa

Specificity: This antibody is specific for the human TCR delta and gamma chains. The T-cell receptor (TCR) is a molecule found on the surface of T cells, or T lymphocytes, that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. On activation through antigen presenting cells (APCs), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant gamma9delta2 (OT3)-Fc fusion protein as an immunogen.

Isotype: IgG2a kappa

Application Details

Application Notes: The binding of this antibody to γδ T cells was determined using flow cytometry. This antibody can compete with commercially available anti-γδ TCR antibody IMMU510 for binding to gamma-delta T cells. It is proposed that, since there is some competition between the two antibodies, G5-4 binds a similar or identical epitope to IMMU510. In an in vitro proliferation assay, this antibody was reported to positively stimulate the proliferation of γδT cells. The scFv version of this antibody was also reported to stimulate the secretion of cytokines like IFN-γ and TGF-α. G5-4ScFv-amplified γδT cells can effectively kill daudi cells, and the killing efficiency is greater than 85 % when the effect-to-target ratio is 5:1 (CN103130894).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Alternative Name: Gamma Delta TCR

Background: TCR γδ, γδ TCR, TCR GD, GD TCR, TCRg/d, T-cell surface glycoprotein CD3 delta chain, CD3D, T3D, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 gamma chain, CD3G, T-cell receptor T3 gamma chain, CD3g, T3G

UniProt: P04234, P09693