Vinculin antibody (pTyr1065, pTyr1133)
This Rabbit Polyclonal antibody specifically detects Vinculin in WB. It exhibits reactivity toward Human, Mouse and Rat.
Quick Overview for Vinculin antibody (pTyr1065, pTyr1133) (ABIN7543992)
Target
See all Vinculin (VCL) Antibodies
Vinculin (VCL)
Reactivity
Human, Mouse, Rat
Host
Rabbit
Clonality
Polyclonal
Conjugate
This Vinculin antibody is un-conjugated
Application
Western Blotting (WB)
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Binding Specificity
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pTyr1065, pTyr1133
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Expression System
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HEK-293 Cells
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Purpose
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Rabbit polyclonal antibody to Vinculin (Phospho-Y1133/1065)
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Specificity
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Recognizes endogenous levels of Vinculin protein only when phosphorylated at Y1133/1065
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Purification
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The antibody was purified by immunogen affinity chromatography.
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Immunogen
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KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1133/1065 of human Vinculin. The exact sequence is proprietary.
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Application Notes
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WB (1/500 - 1/1000)
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Restrictions
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For Research Use only
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Format
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Liquid
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Buffer
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Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
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Preservative
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Sodium azide
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Precaution of Use
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This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
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-20 °C
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Storage Comment
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Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Target
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Vinculin (VCL)
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Alternative Name
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VCL
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Background
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Alternative Names: Vinculin, Metavinculin, MV
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Gene ID
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7414, 22330, 305679
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Pathways
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Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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