BrdU antibody (HRP)

Catalog No. ABIN722670

The HRP-conjugated Mouse Monoclonal anti-BrdU antibody (Clone A7) (ABIN722670) specifically detects BrdU in IHC (p), IHC (fro) and WB.

Quick Overview for BrdU antibody (HRP) (ABIN722670)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to HRP

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Clone: A7

Product Details anti-BrdU Antibody (HRP)

Purpose: BrdU (Proliferation Marker) Monoclonal Antibody, HRP Conjugated

Cross-Reactivity: Various Species

Purification: Purified by Protein G.

Immunogen: BrdU conjugated to KLH

Isotype: IgG

Application Details

Application Notes: WB(1:500-2000), IHC-P(1:500-1000), IHC-F(1:100-500)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical

Background:

Synonyms: Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2_-deoxyuridine, 5-BrdU, proliferation Marker.

Background: Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is incorporated into DNA during S-phase of a cell cycle. It is commonly used in the detection of proliferating cells in living tissues