HA-Tag antibody

Catalog No. ABIN722796

Cited in 2+ publications. The Mouse Monoclonal anti-HA-Tag antibody (Clone 12CA5) (ABIN722796) specifically detects HA-Tag in WB and IF (cc). The antibody is reactive with Tag samples.

Quick Overview for HA-Tag antibody (ABIN722796)

Target: HA-Tag

Reactivity: Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 12CA5

Product Details anti-HA-Tag Antibody

Sequence: YPYDVPDYA

Cross-Reactivity: Tag

Cross-Reactivity (Details): HA tag

Purification: Purified by Protein G.

Immunogen: KLH conjugated to HA tag

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HA-Tag antibody (ABIN722796)

Zhang, Feng, Li, Wang, Li, Sun, Li, Zheng, Liu, Yang, Niu, Zhong, Chen: "Effects of the fusion design and immunization route on the immunogenicity of Ag85A-Mtb32 in adenoviral vectored tuberculosis vaccine." in: Human vaccines & immunotherapeutics, Vol. 11, Issue 7, pp. 1803-13, (2015) (PubMed).

Yang, Wills, Lim, Zhou, Baines: "Association of herpes simplex virus pUL31 with capsid vertices and components of the capsid vertex-specific complex." in: Journal of virology, Vol. 88, Issue 7, pp. 3815-25, (2014) (PubMed).

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA tag

Target Type: Tag

Background: Optional[synonyms]: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.