Nestin antibody (AA 681-750)

Catalog No. ABIN723530

The Rabbit Polyclonal anti-Nestin antibody has been validated for ELISA, FACS, IHC (p), IHC (fro), IF (p) and IF (cc). It is suitable to detect Nestin in samples from Human, Mouse and Rat. There are 6+ publications available.

Quick Overview for Nestin antibody (AA 681-750) (ABIN723530)

Target: Nestin (NES)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Nestin Antibody

Binding Specificity: AA 681-750

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Nestin

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Nestin antibody (ABIN723530)

Wang, Zhang, Chen, Xue, Guo, Liu, Zhao: "Formylpeptide Receptors Promote the Migration and Differentiation of Rat Neural Stem Cells." in: Scientific reports, Vol. 6, pp. 25946, (2018) (PubMed).

Kawashima, Shinkai, Suzuki: "Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping." in: Dental materials journal, Vol. 35, Issue 3, pp. 479-89, (2016) (PubMed).

Liu, Xue, Li, Zhang, Fu, Feng, Chen, Xu: "Amelioration of stroke-induced neurological deficiency by lyophilized powder of catapol and puerarin." in: International journal of biological sciences, Vol. 10, Issue 4, pp. 448-56, (2014) (PubMed).

Shan, Zhou, Yang, Yan, Zhang, Fu, Jiang: "Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/?-catenin signaling." in: Cell biology international, (2014) (PubMed).

Yamamizu, Fujihara, Tachibana, Katayama, Takahashi, Hara, Imai, Shinkai, Yamashita: "Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a." in: Cell stem cell, Vol. 10, Issue 6, pp. 759-70, (2012) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background:

Synonyms: ESTM 46, FLJ 21841, FLJ21841, Intermediate filament protein, Nbla00170, Nes, NEST_RAT, Nestin.

Background: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Gene ID: 25491

UniProt: P21263