Nestin antibody (AA 681-750) (Cy5)

Catalog No. ABIN723534

This anti-Nestin antibody is a Rabbit Polyclonal antibody detecting Nestin in FACS, IF (p) and IF (cc). Suitable for Human, Mouse and Rat.

Quick Overview for Nestin antibody (AA 681-750) (Cy5) (ABIN723534)

Target: Nestin (NES)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is conjugated to Cy5

Application: Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Nestin Antibody (Cy5)

Binding Specificity: AA 681-750

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Nestin

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background:

Synonyms: ESTM 46, FLJ 21841, FLJ21841, Intermediate filament protein, Nbla00170, Nes, NEST_RAT, Nestin.

Background: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Gene ID: 25491

UniProt: P21263