Bcl-2 antibody (AA 101-160)

Catalog No. ABIN723920

The Rabbit Polyclonal anti-Bcl-2 antibody has been validated for WB, FACS, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect Bcl-2 in samples from Human, Mouse, Rat, Rabbit, Cow, Chicken and Bacteria. There are 4+ publications available.

Quick Overview for Bcl-2 antibody (AA 101-160) (ABIN723920)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Chicken, Bacteria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 101-160

Cross-Reactivity: Bacteria, Chicken, Cow, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Bcl-2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Bcl-2 antibody (ABIN723920)

She, Zhao, Zhu, Han, Xia, Bai, Zhang, Li: "Aluminum Trichloride Disorders Bile Acid Secretion and Induces Hepatocyte Apoptosis in Rats." in: Cell biochemistry and biophysics, (2015) (PubMed).

He, Song, Liu, Chen, Hua: "Rapamycin inhibits acrolein-induced apoptosis by alleviating ROS-driven mitochondrial dysfunction in male germ cells." in: Cell proliferation, Vol. 47, Issue 2, pp. 161-71, (2014) (PubMed).

Tian, Zhang, Wang, Dai, Shen, Yang, Huang: "The protective effect of hyperbaric oxygen and Ginkgo biloba extract on A?25-35-induced oxidative stress and neuronal apoptosis in rats." in: Behavioural brain research, Vol. 242, pp. 1-8, (2013) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background:

Synonyms: Bcl-2, Apoptosis regulator Bcl-2, Bcl2

Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Gene ID: 24224

UniProt: P49950

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling