DFFB antibody (AA 201-260)

Catalog No. ABIN724085

The Rabbit Polyclonal anti-DFFB antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect DFFB in samples from Human, Mouse and Rat.

Quick Overview for DFFB antibody (AA 201-260) (ABIN724085)

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DFFB Antibody

Binding Specificity: AA 201-260

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse DFF-40 beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DFFB

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Alternative Name: DFFB

Background:

Synonyms: CAD, CPAN, 40 kDa, DFF40, Didff, 5730477D02Rik, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, Caspase-activated DNase, DNA fragmentation factor 40 kDa subunit, DFF-40, Dffb

Background: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Gene ID: 13368

UniProt: O54788

Pathways: Apoptosis, Caspase Cascade in Apoptosis