APAF1 antibody (AA 1201-1248) (HRP)

Catalog No. ABIN724304

The Rabbit Polyclonal anti-APAF1 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect APAF1 in samples from Human, Rat and Zebrafish (Danio rerio).

Quick Overview for APAF1 antibody (AA 1201-1248) (HRP) (ABIN724304)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APAF1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-APAF1 Antibody (HRP)

Binding Specificity: AA 1201-1248

Cross-Reactivity: Human, Rat, Zebrafish (Danio rerio)

Predicted Reactivity: Mouse,Dog,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Apaf-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: APAF1CT

Background:

Synonyms: CED4, APAF-1, Apoptotic protease-activating factor 1, APAF1, KIAA0413

Background: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Gene ID: 317

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity