AKT 1/2/3 antibody (Biotin)

Catalog No. ABIN7565735

The Rabbit Polyclonal anti-AKT 1/2/3 antibody is suitable to detect AKT 1/2/3 in samples from Human. It has been validated for WB, IHC, FACS, ELISA, IP and FM.

Quick Overview for AKT 1/2/3 antibody (Biotin) (ABIN7565735)

Target: AKT 1/2/3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT 1/2/3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Product Details anti-AKT 1/2/3 Antibody (Biotin)

Purpose: AKT Antibody Biotin Conjugated

Cross-Reactivity (Details): AKT Antibody reacts with the AKT from human tissues.

Characteristics: Synonyms: rabbit anti-AKT Antibody Biotin Conjugated, Biotin conjugated rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1 Antibody, AKT2 Antibody, AKT3 Antibody, Biotin, BAC

Purification: Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Biotin Conjugated AKT Antibody is tested for western blotting to detect a single band of the expected apparent molecular weight. This antibody is suitable for immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below. Flow Cytometry Dilution: User Optimized Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:500 - 1:2,000 Immunoprecipitation Dilution: 1:500 - 1:2,000 ELISA Dilution: User Optimized IF Microscopy Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1, AKT2, AKT3

Background: Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Gene ID: 10000

NCBI Accession: NP_001014431

UniProt: P31749