GATA4 antibody

Catalog No. ABIN7565766

This anti-GATA4 antibody is a Mouse Monoclonal antibody detecting GATA4 in WB and ELISA. Suitable for Human.

Quick Overview for GATA4 antibody (ABIN7565766)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GATA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 10D8-B10-D4

Product Details anti-GATA4 Antibody

Purpose: GATA4 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93 % homology.

Characteristics: Synonyms: mouse anti-GATA4 Antibody, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody

Purification: Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG1 kappa

Application Details

Application Notes: Application Note: Anti-GATA4 antibody has been tested by ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:500-1:1000 ELISA Dilution: 1:2000-1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Alternative Name: GATA4

Background: Background: GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Gene ID: 2626

NCBI Accession: NP_002043

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis