Hexokinase 2 antibody (HRP)

Catalog No. ABIN7565775

This anti-Hexokinase 2 antibody is a Rabbit Polyclonal antibody detecting Hexokinase 2 in WB and ELISA. Suitable for Saccharomyces cerevisiae.

Quick Overview for Hexokinase 2 antibody (HRP) (ABIN7565775)

Target: Hexokinase 2 (HK2)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-Hexokinase 2 Antibody (HRP)

Purpose: Hexokinase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].

Characteristics: Synonyms: rabbit anti-Hexokinase antibody HRP conjugation, peroxidase conjugation rabbit anti-Hexokinase antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody, HEX1, HKB

Purification: Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Hexokinase [Yeast]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-HEXOKINASE Peroxidase Conjugated Antibody has been tested by ELISA and western blot and can be used in applications such as Immunohistochemistry (IHC), and Immunofluorescence (IF). Specific conditions should be optimized by researcher. Western Blot Dilution: 1:500 - 1:1,000 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: HXK2

Background: Background: HEXOKINASE Antibody is specific for hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose. Anti Hexokinase is ideal for investigators involved in glucose energy metabolism, enzymes and AKT signaling research.

Gene ID: 852639

UniProt: P04806

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect