IL-8 antibody

Catalog No. ABIN7565780

The Rabbit Polyclonal anti-IL-8 antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect IL-8 in samples from Human.

Quick Overview for IL-8 antibody (ABIN7565780)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-IL-8 Antibody

Purpose: IL-8 Antibody

Cross-Reactivity (Details): The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA.

Characteristics: Synonyms: Anti-CXCL 8, CXCL8 antibody, 310C antibody, AMCF 1 antibody, AMCF1 antibody, b ENAP antibody, Beta thromboglobulin like protein antibody, CXC chemokine ligand 8 antibody, nterleukin8 antibody, K60 antibody, LECT antibody, LUCT antibody, rabbit anti-Interleukin 8 Antibody, rabbit anti-IL-8

Purification: Anti-IL 8 antiserum has been heated to 56°C for 30 minutes.

Sterility: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: IL 8 antibody has been tested for use in ELISA and western blot. It recognizes IL-8 found in cells, supernatants and other body fluids.  Reactivity in other immunoassays is unknown. Immunohistochemistry Dilution: 1:400 - 1:800 Western Blot Dilution: 1:500 - 1:2,000 Immunoprecipitation Dilution: 1:400 - 1:800 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 75 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL8

Background: Background: Interleukin-8 is a chemokine produced by macrophages and other cell types such as epithelial cells. Human IL-8 (CXCL8) antibody is commonly used in many experiments focused around inflammation. Anti-IL-8 antibody is ideal for investigators involved in Cancer, Immunology and Cardiovascular research.

Gene ID: 3576

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy