Serum antibody (HRP)

Catalog No. ABIN7565800

This Goat Polyclonal antibody specifically detects Serum in ELISA, WB, IP, IHC, DB, FLISA and FM. It exhibits reactivity toward Mouse.

Quick Overview for Serum antibody (HRP) (ABIN7565800)

Target: Serum

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Dot Blot (DB), FLISA, Fluorescence Microscopy (FM)

Product Details

Purpose: Mouse Serum Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.

Characteristics: Synonyms: goat anti-Mouse Serum Antibody Peroxidase Conjugated, goat anti-Mouse Serum HRP Conjugated Peroxidase

Purification: Anti-Mouse serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Mouse serum proteins

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Mouse Serum Peroxidase Conjugated Antibody has been tested by dot blot and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Anti-MOUSE SERUM (Goat) Antibody is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. Specific conditions should be optimized by user. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized Immunoprecipitation Dilution: User Optimized FLISA Dilution: User Optimized ELISA Dilution: User Optimized IF Microscopy Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Serum

Background: Background: Anti-Mouse Serum antibody detects mouse serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Mouse Serum antibody is ideal for investigators involved in Cell Signaling, cellular biology and Signal Transduction research.