RFP antibody

Catalog No. ABIN7565834

The Goat Polyclonal anti-RFP antibody has been validated for WB, ELISA and FM. It is suitable to detect RFP in samples from Discosoma.

Quick Overview for RFP antibody (ABIN7565834)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Product Details anti-RFP Antibody

Purpose: RFP Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma).

Characteristics: Synonyms: goat anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification: Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: Polyclonal anti-RFP has been tested by western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27 kDa in Western Blot in the appropriate cell lysate or extract. Western Blot Dilution: 1:1000-1:10,000 ELISA Dilution: 1:2,000 - 1:10,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: DsRed

Background: Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS).

UniProt: Q9U6Y8