Liver Arginase antibody (HRP)

Catalog No. ABIN7565855

The Rabbit Polyclonal anti-Liver Arginase antibody has been validated for WB, ELISA and DB. It is suitable to detect Liver Arginase in samples from Cow.

Quick Overview for Liver Arginase antibody (HRP) (ABIN7565855)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Liver Arginase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Product Details anti-Liver Arginase Antibody (HRP)

Purpose: Arginase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver].

Characteristics: Synonyms: rabbit anti-Arginase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Arginase Antibody, A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody

Purification: Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Arginase [Bovine Liver]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Arginase Peroxidase Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Arginase [Bovine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:40,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: ARG1

Background: Background: Arginase is a manganese-containing enzyme belonging to the ureohydrolase family of enzymes. Arginase catalyzes the fifth and final step in the urea cycle, a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. Specifically, arginase converts L-arginine into L-ornithine and urea.

Gene ID: 513608

NCBI Accession: NP_001039619

UniProt: Q2KJ64

Pathways: Cellular Response to Molecule of Bacterial Origin