BLRO_MYRVE antibody (HRP)

Catalog No. ABIN7565861

The Goat Polyclonal anti-BLRO_MYRVE antibody is suitable to detect BLRO_MYRVE in samples from Myrothecium verrucaria. It has been validated for ELISA, IP and WB.

Quick Overview for BLRO_MYRVE antibody (HRP) (ABIN7565861)

Target: BLRO_MYRVE

Reactivity: Myrothecium verrucaria

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Purpose: Bilirubin Oxidase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].

Characteristics: Synonyms: goat anti-Bilirubin Oxidase HRP conjugation, goat anti-Bilirubin Oxidase Antibody Peroxidase Conjugated

Purification: Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Bilirubin Oxidase has been tested by western blot and is suitable to be assayed against 1.0 μg of Bilirubin Oxidase [Myrothecium verrucaria] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: BLRO_MYRVE

Background: Background: Bilirubin Oxidase is key in the oxidation of bilirubin and other tetrapyrroles. This enzyme belongs to the family of oxidoreductases, to be specific those acting on the CH-CH group of donor with oxygen as acceptor. The systematic name of this enzyme class is bilirubin:oxygen oxidoreductase. This enzyme is also called bilirubin oxidase M-1. This enzyme participates in porphyrin and chlorophyll metabolism.

UniProt: Q12737