Catalase antibody (HRP)

Catalog No. ABIN7565869

This Rabbit Polyclonal antibody specifically detects Catalase in WB and ELISA. It exhibits reactivity toward Cow.

Quick Overview for Catalase antibody (HRP) (ABIN7565869)

Target: Catalase (CAT)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Catalase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-Catalase Antibody (HRP)

Purpose: Catalase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine Liver].

Characteristics: Synonyms: rabbit anti-Catalase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody

Purification: Catalase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Catalase [Bovine Liver]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Catalase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Catalase [Bovine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500- 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: CAT

Background: Background: Catalase is found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species.

Gene ID: 531682

UniProt: P00432

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism