CELA1 antibody (HRP)

Catalog No. ABIN7565876

Product Details anti-CELA1 Antibody (HRP)

Target: CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CELA1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Elastase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas].

Characteristics: Synonyms: rabbit anti-Elastase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

Purification: Elastase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Elastase [Porcine Pancreas]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Elastase Peroxidase conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Elastase [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CELA1

Target: CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

Alternative Name: CELA1 (CELA1 Products)

Background: Background: Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. Elastase breaks down elastin, an elastic fiber that, together with collagen, determines the mechanical properties of connective tissue. Elastase also has the important immunological role of breaking down Shigella virulence factors. Elastase is responsible for pulmonary emphysema, cyclic hematopoiesis, and blistering in bullous pemphigoid.

Gene ID: 396766

NCBI Accession: NP_998988

UniProt: P00772